Geminivirus Activates ASYMMETRIC LEAVES 2 to Accelerate Cytoplasmic DCP2-Mediated mRNA Turnover and Weakens RNA Silencing in Arabidopsis
Fig 6
Decapping machinery antagonizes PTGS.
(A) AS2 promotes DCP2 decapping activity in vitro. Addition of BV1 can further increase the ability of AS2 to enhance the decapping activity of DCP2. (B) In vivo AtEXPL1 mRNA stability of as2-1 mutant and AS2 complementation lines after cordycepin treatment. In WT (Col-0) plants, the half-life(½t) for AtEXPL1mRNA was 40 min. The level of AtEXPL1 mRNA in each time point was normalized against that of solvent (DMSO) treatment. Actin2 was used as an internal control. Values are mean ± SD (n = 3 biological replicates). (C) Relative virus titer of AS2 complementation lines infected with CaLCuV. Values are mean ± SD (n = 3 biological replicates).