Goblet Cell Derived RELM-β Recruits CD4+ T Cells during Infectious Colitis to Promote Protective Intestinal Epithelial Cell Proliferation
Fig 5
Reduced mucosal CD4+ T-cell numbers within infected Rag1-/- and Retnlb-/- mice, leading to defective epithelial cell proliferation.
(A) CD4 staining in the colons of infected Rag1-/- mice reconstituted with either PBS or CD4+ T cells. (B) Ki67 staining in PBS or CD4+ T cell-reconstituted Rag1-/- mice (10 DPI). Graph on right (B1): Enumeration of Ki67-labeled cells. Bars represent mean average number of Ki67+ cells/100 cells. Error bars = SD. *P < 0.01. (C) LPS staining showing deeper penetration of C. rodentium into the crypts of PBS-reconstituted Rag1-/- mice (arrows) as compared to CD4+ T cell-reconstituted Rag1-/- mice (10 DPI). Scale bar = 100 μm. Original magnification = 200X. (D) CD4 staining in uninfected or 10 DPI C57BL/6 and Retnlb-/- mice. (E) Enumeration of CD4+ cells within colonic sections. Results represent means of cells counted from 5–10 high power fields (400X)/mouse. *P < 0.05. Error bars = SEM. (F) Flow cytometry of lamina propria lymphocytes purified from infected C57BL/6 and Retnlb-/- mice (8 DPI). All results are representative of 2–3 independent experiments, with 3–5 mice/group.