Novel Disease Susceptibility Factors for Fungal Necrotrophic Pathogens in Arabidopsis
Fig 6
PROVIR1-to-13 overexpression confers enhanced disease susceptibility to P. cucumerina infection.
(A) Western blots with anti-GFP antibodies of crude protein extracts derived from T3 homozygous Col-0 plants expressing either a 35S::GFP construct or the respective PROVIR1-to-13::GFP constructs. Two independent lines for each PROVIR::GFP construct were used and the accumulation of the encoded fusion protein was compared to that of free GFP. Equal protein loading was check by Ponceau staining of the nitrocellulose filter. (B) Resistance response to P. cucumerina of Col-0 and two independent homozygous lines expressing each of the respective PROVIR::GFP proteins shown in panel A. Disease was evaluated 11 d.p.i. by determining the average lesion diameter on three leaves per plant from 15 plants per genotype. Data points represent average lesion size ± SE of measurements. An ANOVA was conducted to assess significant differences in disease symptoms, with a priori P < 0.05 level of significance; significant differences are indicated with letters. (C) Accumulation levels of endogenous PROVIR7, PROVIR9, PROVIR12, and GRP3 transcripts measured in comparatively healthy Col-0 plants (left bars) and in T-DNA provir7, provir9, provir12-1, provir12-2, and grp3 T-DNA insertion mutants (right black bars). Data represent mean ± SD; n = 3 biological replicates. Expression was normalized to the constitutive ACT2 gene, then to expression in Col-0 plants. (D) Resistance response to P. cucumerina of Col-0 and provir7, provir9, provir12-1, provir12-2, and grp3 plants. Disease was evaluated as in B.