Programmed Ribosomal Frameshift Alters Expression of West Nile Virus Genes and Facilitates Virus Replication in Birds and Mosquitoes
Figure 4
Infection of WT and IRF3−/−7−/− MEFs with PRF-deficient WNVKUN viruses induces differential expression of several genes.
Low passage primary WT and IRF3−/−7−/− MEFs were infected at MOI of 1 with WNVKUN WT, WNVKUN A30A′, or WNVKUN A30P viruses and 48 hpi total RNA was extracted and used for analysis of global gene expression by microarrays. (A) Virus titres in the infected cell culture supernatants were assayed by plaque assay at 0 and 48 hpi. (B) Heat map showing top differentially expressed genes ranked by log fold change between different experimental conditions.