Identification and Characterisation of a Hyper-Variable Apoplastic Effector Gene Family of the Potato Cyst Nematodes
Figure 6
HYP effectors are expressed in the amphid sheath cells of parasitic females.
A) Schematic representation of Globodera pallida feeding female [64]. In situ hybridisation probe designed to a conserved region common to all HYP effectors identifies extremely strong and specific dark staining in a paired structure anterior to the metacorpal bulb of 14 days post infection females (C). No such staining pattern is observed with the negative control, or the positive control on second stage juvenile (J2) nematodes (B). Comparison of the staining pattern with a schematic identified this structure as the two lobed amphid sheath cells.