Dengue Viruses Are Enhanced by Distinct Populations of Serotype Cross-Reactive Antibodies in Human Immune Sera
Figure 1
Primary DENV-immune human sera enhance heterotypic but not homotypic serotypes at high serum concentrations in vitro and in vivo.
(A and B) Enhancement of heterotypic and homotypic DENV by primary DENV2-immune (DT 001) (A) and DENV3-immune (DT118) (B) sera at high serum concentration (1∶40) in K562 cells. Data are representative of 8 serum samples. C) Human serum was passively transferred to AG129 mice 24 hours prior to administration of 2×105 pfu of the DENV2 strain D2S10 and survival of the mice was assessed. D) ADE-induced mortality in AG129 mice after D2S10 infection in the presence of heterotypic sera (primary DENV1, primary DENV3, and primary DENV4 sera) and homotypic human serum (primary DENV2 serum). Statistics: in Figure 1A, ADE of DENV2 compared to DENV1, *p = 0.01; DENV3, **p<0.001; and DENV4, ***p<0.0001; in Figure 1B, ADE of DENV3 compared to DENV1, *p = 0.02; DENV2, **p<0.005; and DENV4, ***p = 0.0003; in Figure 1D, NHS compared to anti-DENV1 serum, ***p = 0.0007; anti-DENV3 serum, ****p<0.0001; anti-DENV4 serum, ****p<0.0001.