Incomplete Deletion of IL-4Rα by LysMCre Reveals Distinct Subsets of M2 Macrophages Controlling Inflammation and Fibrosis in Chronic Schistosomiasis
Figure 1
Surviving chronic S. mansoni infection depends on Il4rα allele, not LysMCre expression.
IL-4Rαflox/ΔLysMCre mice (open circles and bars), IL-4Rαflox/Δ littermate controls (solid circles and bars), IL-4Rαflox/flox mice (solid squares), and IL-4RαΔ/Δ (open triangles) were infected percutaneously with 35 Schistosoma mansoni cercariae. A. Survival kinetics through 16 weeks (n = 10–20 per group). B. Th1 response. 9 or 16 weeks post-infection, liver leukocytes were isolated, restimulated with phorbol myristate acetate/ionomycin, stained for IFN-γ, and analyzed by flow cytometry (n = 7–15, ns = not significant). C. Hepatotoxicity. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were assayed in serum 9 or 16 weeks after infection (n = 7–8 mice, ns = not significant). Data shown are mean ±SEM and represent at least two independent experiments.