Neutrophil Elastase Causes Tissue Damage That Decreases Host Tolerance to Lung Infection with Burkholderia Species
Figure 3
Neutrophil elastase reduces host tolerance to infection with B. pseudomallei.
Mice (n = 5) were intranasaly infected with 400 CFU B. pseudomallei and their survival and weight were monitored (A). (B) Flow cytometric analysis of BAL cells and levels of MPO in BALF collected 72 h p.i. from mice (n = 5) infected as in A. (C) Representative lung sections stained with H&E and the total area of the inflammatory nodules was measured and expressed as percentage of the total lung lobe area (n = 5). (D) Tissue burdens of B. pseudomallei at 72 h p.i. Data are expressed as mean + S.D. *p<0.05. (A) log rank Kaplan-Meier test, (B–D) Mann-Whitney U test.