Capsid Protein VP4 of Human Rhinovirus Induces Membrane Permeability by the Formation of a Size-Selective Multimeric Pore
Figure 9
HRV-induced permeability is size-selective.
Liposomes containing FITC-labelled dextrans of 4 kD (FD4), 10 kD (FD10), 70 kD (FD70) or 250 kD (FD250) were mixed with 0.5 µg HRV or preheated HRV. Release of dextrans was quantified by pelleting the liposomes and measuring the fluorescence in the supernatant (as in figure 4). Data is presented as percentage of total release observed by lysis of liposomes by addition of detergent. Error bars represent standard error of the mean (n = 3) and asterisks indicate statistical significance calculated by one way Anova (p*<0.05). Data is representative of three independent experiments.