Genetic Analysis of Leishmania donovani Tropism Using a Naturally Attenuated Cutaneous Strain
Figure 3
Chromosome copy number comparison between the VL-SL and CL-SL strains (Panel A).
Read depth was scaled to give a value of 2 for disomic chromosomes. Comparison of chromosome transcript levels in the VL-SL and CL-SL strains (Panel B). Total RNA was prepared from promastigotes, axenic amastigotes and macrophages infected with the VL-SL and CL-SL strains. The cDNA libraries prepared from the RNAs were subjected to SL-sequencing and the cDNA coverage from each chromosome was normalized to the entire genome. Pro, axenic promastigotes; Ax, axenic amastigotes; Am, infected macrophage-derived amastigotes.