IscR Is Essential for Yersinia pseudotuberculosis Type III Secretion and Virulence
Figure 1
Alignment of protein sequences shows a high level of conservation between E. coli and Yersinia IscR.
(A) The Y. pseudotuberculosis DNA sequence, which displays the unique insertions sites for the two transposon mutants generated from our genetic screen. A space in the DNA sequence and a solid black line indicate the site of insertion for either iscR::Tn1 or iscR::Tn2. (B) Multiple sequence alignment was performed on the IscR protein sequence from E. coli K12-MG1655 and each of the three human pathogenic Yersinia spp., Y. pseudotuberculosis IP 32953 (Y. pstb), Y. enterocolitica 8081 (Y. ent) and Y. pestis CO92 (Y. pestis) using ClustalW [86]. The N-terminal helix-turn-helix DNA-binding motif is indicated by a black box. The three conserved cysteine residues (C92, C98 and C104) responsible for coordinating an Fe-S cluster are in bold and identified by black arrows [33]. Asterisks indicate nucleotides that are conserved across all four species.