Molecular Signatures of Hemagglutinin Stem-Directed Heterosubtypic Human Neutralizing Antibodies against Influenza A Viruses
Figure 3
Studying the location of SHM hotspots and nucleotide substitutions frequencies in the IGHV1-69 reference Ab dataset.
A) Number of V-segment substitutions observed in 38 HV1-69-sBnAbs with color notations that designate the HV1-69-sBnAbs characterized by the distinctive CDR-H2 Ser52 or Ala/Gly52a and “other” which do not contain CDR-H2 Ser52 or Ala/Gly52a. (B–D) Upper panel – the common nucleotide substitutions that generated the distinctive amino acid substitutions in the respective CDR domain. Main panel - The IGHV1-69 51p1 Ab reference dataset was studied for substitution frequency of nucleotides in the V-segment CDRs and for location of AID and polη hotspots (AID = WRCY yellow solid triangle/RGYW dark red solid triangle, Polη WA brown open triangle/TW blue empty triangle. The horizontal line shows the mean±SD (6.44±7.23) of non-germline nucleotide substitution frequency observed for FR regions to serve as a reference.