Intracellular Theileria annulata Promote Invasive Cell Motility through Kinase Regulation of the Host Actin Cytoskeleton
Figure 6
MAP4K4 promotes lamellipodium formation and enhances ERM accumulation in lamellipodia.
A) Confocal IFA of endogenous MAP4K4 (green), F-actin (red) and TaSP (blue) in cells transfected with siControl or siMAP4K4. Bar diagram shows quantification of percent cells displaying a large, single lamellipodium in the absence (black) or presence (white) of 25 ng/ml TNFα. C: siControl; 1: siMAP4K4_1; 2: siMAP4K4_2 B) IFA of total ERM (anti-ERM ab) proteins and ERM proteins phosphorylated on C-terminal threonine (anti-pERM ab) in cells seeded on Fn. a and b show 4× magnifications of boxed areas for ERM (a) and pERM (b) distribution. F-actin is in red, ERM and pERM in green, DNA in blue. C) IFA of ERM localization in siControl or siMAP4K4 transfected cells. IFA as in B, c and d show 4× magnifications of boxed areas for ERM accumulation in lamellipodia of siControl (c) and or siMAP4K4 transfected cells (d). See also Figure S6.