Cytomegalovirus m154 Hinders CD48 Cell-Surface Expression and Promotes Viral Escape from Host Natural Killer Cell Control
Figure 1
MCMV infection leads to reduced expression of SLAM receptors on the macrophage surface.
(A) Surface expression of the SLAM receptors CD48, CD84, CD150, CD229, CD244, and Ly108 on peritoneal macrophages was tested by flow cytometry using specific mAbs against each of these molecules (black line histograms). Isotype IgGs directly conjugated with PE or APC were used as negative controls (dashed line histograms). A representative experiment out of three performed is shown. (B) Peritoneal macrophages were mock-infected or infected with MCMV-GFP at an moi of 2 and analyzed by flow cytometry at 24, 48 and 72 hours after infection for surface expression of CD48, CD84, CD229, Ly108, and MHC class I (MHC I) as indicated in A. Open histograms represent the expression of these molecules on MCMV-infected (GFP-positive) cells, and shaded histograms represent the expression on uninfected (GFP-negative) cells from the same culture.