KSHV 2.0: A Comprehensive Annotation of the Kaposi's Sarcoma-Associated Herpesvirus Genome Using Next-Generation Sequencing Reveals Novel Genomic and Functional Features
Figure 1
High-resolution mapping of KSHV genomic features.
(A–B) Strategy for identification of transcripts and ribosome footprints in iSLK-219 cells. Latent iSLK-219 cells were induced to enter the lytic cycle by expression of the doxycycline-inducible transcription factor RTA for 8, 24, 48 and 72 hr. mRNA and ribosome footprints were isolated from cells and the purified RNA was deep sequenced. (C) Ribosome occupancy and mRNA profiles of the late lytic gene K8.1 at 48 hr post induction (hpi).