Mediated Plastid RNA Editing in Plant Immunity
Figure 1
Functional OCP3 resides in the chloroplast.
(A) OCP3 amino acid sequence. The 60 amino acid residues conforming the homeodomain are indicated in red letters. The N-terminal signal peptide sequence to chloroplast targeting, as predicted by TargetP, is indicated in green letters. Two canonical bipartite nuclear localization signals (RK-(X)10-KKNKKK and KK-(X)10-RRSKR) are underlined in blue. (B) Fluorescent confocal microscopy evaluation of protein localization in transgenic Arabidopsis plants transformed with a 35S::YFP-OCP3 construct (upper panel) and a 35S::OCP3-YPF constructs (lower panel). YFP-specific fluorescence is shown in green and chlorophyll-derived fluorescence is shown in red. (C) Western blot analysis using anti-GFP antibodies of crude protein extracts derived from Arabidopsis plants transformed with 35S::YFP, 35S::YFP-OCP3 and 35S::OCP3-YPF genes constructs, respectively. Molecular mass markers are shown on the left. (D) Characteristic GUS expression pattern, as driven by the Ep5C gene promoter, in leaves of the ocp3 mutant. Complementation of this molecular phenotype in ocp3 plants upon transformation with 35S::OCP3-YFP but not upon transformation with 35S::YFP-OCP3. (E) Fluorescent confocal microscopy evaluation of protein localization in transgenic ocp3 plants transformed with a 35S::OCP3-YPF construct.