A Comprehensive Analysis of In Vitro and In Vivo Genetic Fitness of Pseudomonas aeruginosa Using High-Throughput Sequencing of Transposon Libraries
Figure 8
Confirmation of the lack of a bottleneck accounting for enhanced fitness of strains with oprD Tn-insertions in the spleen.
A: RPKM reads in six strains with Tn insertions in unannotated genes recovered from the spleen in the original saturated Tn-insertion library reflecting three strains recovered from the spleens and three with <0.1 RPKM reads. B: Proportion of six P. aeruginosa PA14 Tn-insertion strains recovered from the spleen of GI colonized and neutropenic mice in comparison to Tn-insertion strain PA14 Tn-oprD. Bars represent the means, error bars the S.D.