Host Factor SAMHD1 Restricts DNA Viruses in Non-Dividing Myeloid Cells
Figure 4
HIV-1 RT primer extension assay monitors dNTP levels.
(A) MDM sample extracts were analyzed using a dNTP assay. 5′ 32P-end-labeled primer (“P”) was individually annealed to one of four different templates. The molar amount of the nucleotide extension product (“P+1”) is equal to that of each dNTP contained in the extracted samples, which allows us to calculate and compare the dNTP concentrations for the different treatments [13]. Representative gels are shown for one of three MDM donors monitoring the levels of dNTPs under various conditions. The lane key indicates the different treatment conditions used for each lane. (B) Quantitation of the raw data was plotted as bar graphs for each of the dNTP concentrations. Fold changes in dNTPs between different MDMs treatment groups were plotted.