Evasion of Antiviral Innate Immunity by Theiler's Virus L* Protein through Direct Inhibition of RNase L
Figure 5
TMEV L* and muRNase L proteins interact in infected murine cells.
L929 cells stably expressing FLAG-muRNase L were infected with FS97, a recombinant TMEV coding for HA-L*. Cell lysates were prepared and immunoprecipitated with indicated antibodies (IP:FLAG or IP:HA). A. Immunocomplexes were analyzed by Western blotting with the indicated antibodies (WB:HA or WB:FLAG). Arrows point to the coimmunoprecipitated proteins. B. As a control cell lysates corresponding to 5% of the input used for immunoprecipitations were analyzed by immunoblot using the same antibodies as in A. IgH, immunoglobulin heavy chain; IgL, immunoglobulin light chain.