Extreme Resistance as a Host Counter-counter Defense against Viral Suppression of RNA Silencing
Figure 7
VSRs that bind small RNAs in vitro do not compromise resistance mediated by Rx against PVX.
Leaves of five week-old N. tabacum expressing the Rx gene and its corresponding counterpart lacking this R gene was infiltrated with of A. tumefaciens strains expressing PVX-GFP together with a strain expressing P14, P15, P21, P25, HcPro or the GUS reporter gene. GFP accumulation in infiltrated leaves was detected by Western analysis using an anti-GFP antibody. Ponceau staining of the membrane is shown to demonstrate equal protein loading. Experiments were repeated three times and gave similar results.