IL-27 Receptor Signalling Restricts the Formation of Pathogenic, Terminally Differentiated Th1 Cells during Malaria Infection by Repressing IL-12 Dependent Signals
Figure 1
WSX-1 signalling restrains the Th1 response during malaria infection.
WT and WSX-1−/− mice were infected i.v. with 104 with P. berghei NK65 pRBC. (A) Representative plots showing T-bet expression by splenic CD4+ effector (CD44+ CD62L−) T cells from naïve and infected WT and WSX-1−/− mice. (B, C) The (B) frequencies and (C) total numbers of splenic CD4+ effector T-bet+ T cells in WT and WSX-1−/− mice. (D) Representative plots showing IFN-γ and TNF expression by splenic Th1 effector CD4+ T cells from naïve and infected WT and WSX-1−/− mice following in vitro PMA and ionomycin stimulation. (E–F) The Mean fluorescence intensity of (E) TNF and (F) IFN-γ expression by CD4+ effector Th1 cells from naïve and infected WT and WSX-1−/− mice. (G) The frequencies of polyfunctional CD4+ effector Th1 cells expressing IFN-γ and TNF within the spleen of naïve and infected WT and WSX-1−/− mice. The results are the mean +/− SEM of the group with 3–5 mice per group. The results are representative of 5 independent experiments. * P<0.05 between WT and WSX-1−/− mice.