ATM and ATR Activities Maintain Replication Fork Integrity during SV40 Chromatin Replication
Figure 5
ATR is crucial for SV40 chromatin replication.
(A) Scheme for application of ATRi during phases of a 48 h SV40 infection. (B) Southern blot of DNA replicated in BSC40 cells when ATRi was present during phases of a 48 h SV40 infection described in (A). (C) Graph of total viral or SV40 monomer DNA signals normalized to SV40 DNA replicated in the presence of DMSO from southern blots as shown in (B). (D, E) Graph of of monomer (D) or aberrant (E) structure(s) accumulated as a result of ATR inhibition from southern blots as shown in (C). Each bar in (C–E) shows the average from 3 to 4 independent experiments.