Arabidopsis Actin-Depolymerizing Factor-4 Links Pathogen Perception, Defense Activation and Transcription to Cytoskeletal Dynamics
Figure 4
Phosphorylation of ADF4 is required for RPS5 mRNA expression.
(A) Western blot of isoelectric focusing (IEF) and SDS PAGE analysis of wild type ADF4 (upper) and phospho-null ADF4_S6A (lower). Arrows indicate direction of IEF and SDS PAGE. (B) The relative expression levels of RPS5 were determined by qRT-PCR. (C) HR phenotypes at 22 hours after bacterial infiltration (upper), disease phenotypes at 4 dpi (lower). (D) Enumeration of bacterial growth at 0 and 4 dpi. HR and bacterial population experiments were repeated at least 3 times. Error bars, representing mean ± SEM, were calculated from two (A; n = 4) or three (D; n = 9) technical replicates of two independent biological repeats. Statistical significance was determined using two-way ANOVA, comparing adf4 to Col-0, with Bonferroni post test, where *p<0.05; ***p<0.001. hpi = hours post inoculation; dpi = days post inoculation.