Glycoprotein N of Human Cytomegalovirus Protects the Virus from Neutralizing Antibodies
Figure 4
Monoclonal antibodies to gB, gH and gN neutralize the gN-truncated viruses more efficiently than the parent virus.
Virus and antibody were incubated for 1 h at 37°C and the mixture was added to HFF monolayers. Cells were washed 4 h later and percent infected cells was determined at 24 h post infection by indirect immunofluorescence using an antibody specific for IE-1. Target proteins and the corresponding mab are indicated. Every mab was tested at least two times with similar results. Data were analyzed by 2way ANOVA and Bonferroni posttests. * p<0.05, **p<0.01, ***p<0.001.