A GATA Transcription Factor Recruits Hda1 in Response to Reduced Tor1 Signaling to Establish a Hyphal Chromatin State in Candida albicans
Figure 1
brg1 is the only mutant found to be defective in Hda1 promoter recruitment.
(A). ChIP of Hda1-Myc in mutants defective in hyphal elongation. Wild type and indicated mutant cells carrying Hda1-Myc were diluted into YPD+10 nM rapamycin medium at 37°C for 6 h. ChIP DNA was quantitated as described [18] by qPCR with primers at the UAS region of HWP1 [30]. The 0 h values of wild type cells were set to be 1.00. The ChIP data show the average of three independent qPCR experiments with error bars representing the SEM. (B) Western analysis of Nrg1-Myc. brg1, rob1, and ahr1 mutant cells carrying Nrg1-Myc were diluted into YPD medium at 37°C for 30 min. (C). Overnight cultures of wild type (SN250) and brg1 mutant were diluted 1∶250 fold into YPD medium at 37°C. 5 nM rapamycin was added after 1 hour to YPD medium. Cells were collected at 0 h, 1 h, and 6 h for cell morphology analysis.