Msb2 Shedding Protects Candida albicans against Antimicrobial Peptides
Figure 4
Msb2 synthesis protects C. albicans against LL-37.
A. Basal LL-37-resistance of C. albicans depends on Msb2. The indicated strains were incubated with different LL-37 amounts for 1.5 h at 37°C before plating of cells to determine colony-forming units (CFUs). Standard deviations of triplicate measurements are indicated; statistical differences of mutant versus control strain cfu values were evaluated by a t-test. B. Staining of C. albicans by TAMRA-labelled LL-37. 50 µl of cells were resuspended in PBS were incubated for 5 min with 5 µg LL-37-TAMRA before visualization using phase contrast and fluorescence microscopy. CAF2-1. +/+; FCCa27, −/−; ESCa3, −/−[+]. C, D. LL-37 sensitivity of C. albicans strains producing Msb2 variants (C) and of pmt mutants producing undeleted Msb2 (D). Transformants producing variant Msb2 proteins are described in Figures 2 and 3. Strain suspensions (5 µl) were co-incubated with 2 µg LL-37 for 1.5 h before CFU determination. Means and standard deviations of triplicate assays are shown. Statistical significance using a t-test is indicated by * (p<0.05), ** (p<0.01) and *** (p<0.001).