SH3 Domain-Mediated Recruitment of Host Cell Amphiphysins by Alphavirus nsP3 Promotes Viral RNA Replication
Figure 7
Deletion of the SH3 binding motif impairs amphiphysin-2 recruitment to RCs, and delays CPV formation.
HeLa cells were infected with SFV wild-type (A) or SFV ΔP1+2 (B) at m.o.i. 50, fixed at the indicated time points and stained with specific antibodies. Colocalization of RCs (dsRNA staining) and amphiphysin-2 is shown in yellow. The scale bars are 10 µm. (C) Deletion of SH3 binding motif impaired amphiphysin-2 recruitment to replication complexes, as indicated by Pearson's coefficients measured for colocalization of amphiphysin-2 with dsRNA and nsP3 in CPVs at 10 h p.i. (n = 8-13 fields examined (at least 34 cells); *p<0.05; ** p<0.005).