EBV Tegument Protein BNRF1 Disrupts DAXX-ATRX to Activate Viral Early Gene Transcription
Figure 1
BNRF1 binds the cellular protein Daxx.
(A) Colloidal blue stained SDS-PAGE of FLAG-immunoprecipitated BNRF1 and interacting partners. 293T cells were stably transfected with empty FLAG vector (V) or FLAG-tagged BNRF1 (B). Cell lysates were subject to Immunoprecipitation (IP) by anti-FLAG antibodies, then analyzed by SDS-PAGE. Bands unique to lane B were cut out and identified by LC/MS/MS. (B) IP confirmation of BNRF1/Daxx interaction. 293T cells were transiently transfected with empty vector (V) or wild-type BNRF1 (B). Cells harvested two days post-transfection were subject to IP with non-specific IgG, anti-FLAG or anti-Daxx antibodies, and analyzed by Western blot (WB) with anti-FLAG or anti-Daxx antibodies. (C) Summary of LC/MS/MS data from FLAG-BNRF1 purification. Genebank accession number (GI), percent of peptide coverage, number of peptides identified, and protein name are indicated.