Multiple Helminth Infection of the Skin Causes Lymphocyte Hypo-Responsiveness Mediated by Th2 Conditioning of Dermal Myeloid Cells
Figure 4
Multiple infections induce a Th2-type cytokine environment in the skin and induce the expression of markers of alternative activation.
(A) Cytokine production by skin biopsies from 1x and 4x mice taken at different days post-infection. Bars show mean cytokine + SEM (n = 6). (B) Analysis of mRNA transcript levels from 1x and 4x DEC collected on day 4 post-final infection defined by qRT-PCR. Data are shown in arbitrary units (a.u.) + SEM relative to the expression of GAPDH for each sample (n = 5). (C) Transcript levels of DEC sorted into regions R2-R4 on the basis of F4/80 and MHC-II. Data are means + SEM of 3–4 separate experiments using DEC populations (n = 15 mice). Significances are shown between groups indicated by connector bars, of naïve or 4x mice compared to 1x mice.