Crystal Structure and Size-Dependent Neutralization Properties of HK20, a Human Monoclonal Antibody Binding to the Highly Conserved Heptad Repeat 1 of gp41
Figure 9
Size dependent neutralization breadth and potency of HK20 in different HIV-1 neutralization assays and comparison with T-20.
(A) Neutralization of 45 HIV-1 isolates in TZM-bl cells by HK20 IgG (empty circles), Fab (red circles) and scFv (black circles). Shown are nM IC50 values. (B) Neutralization of 27 clade B and 25 clade C HIV-1 isolates in TZM-bl cells by HK20 scFv. Shown are nM IC50 values. Two-tailed P value was calculated with the unpaired t test. (C) HK20 scFv (black circles) and T-20 (empty triangles) were tested for their capacity to neutralize 20 HIV-1 isolates using either TZM-bl (left panel) or HOS (right panel) as target cells. Shown are nM IC50 values. The dotted line indicates the lowest concentration tested. (D) HK20 scFv (black circles), TriMab (2F5, 2G12 and b12, empty squares) and T-20 (empty triangles) were tested in a PBMC-based neutralization assay against a panel of 9 HIV-1 primary isolates from 5 different clades. Shown are nM IC90 values. The dotted lines indicate the highest concentration tested. Virus isolates used and detailed results are shown in Tables S1, S2, S3, S4, S5 in Supporting Information S1.