The Combined Effect of Environmental and Host Factors on the Emergence of Viral RNA Recombinants
Figure 3
Increased stability of the TBSV repRNA in met22Δ yeast.
(A) The DI-72 repRNA was expressed in both BY4741 parental strain and met22Δ strain from the GAL1 promoter (in the absence of p92), followed by suppression of repRNA synthesis by glucose. Total RNA samples were analyzed at various time points (as shown) by Northern blotting using a DI-72(+) repRNA-specific probe. The ribozyme cleaved repRNA was quantified and the half-life of the repRNA is shown in minutes. (B) Similar profile of recRNAs and degRNAs in double deletion and in xrn1Δ yeasts. Northern blot analysis was performed on total RNA samples from the shown yeast strains replicating DI-72 repRNA. See further details in Fig. 1.