CD14 Signaling Restrains Chronic Inflammation through Induction of p38-MAPK/SOCS-Dependent Tolerance
Figure 2
CD14 deficiency is associated with greater inflammation and higher bacterial burden with reduced socs transcription in response to B. burgdorferi.
A) CD14+/+ and CD14−/− mice were infected with 1×105 B. burgdorferi and tibiotarsal joint thickness was measured at 1 week intervals. The horizontal bars indicate mean thickness for each group and the data are representative of two independent experiments (n = 24). B) Total RNA was isolated from the tibiotarsal joints of CD14+/+ and CD14−/− mice (n = 6), an equal amount of RNA was pooled from each joint and 0.5 µg of pooled RNA was used for preparing cDNA. The cDNA was analyzed by qPCR to determine the levels of irf1, socs1 and socs3 transcript. Results represent mean±SEM from two independent experiments wherein samples were run in triplicate. *P<0.05, **P<0.01. C) DNA from the indicated organs was collected 6 weeks p.i. and the bacterial burden was determined using Taqman® probes for flaB of B. burgdorferi; murine nidogen served as a control. Results represent mean±SEM from two independent experiments (n = 24) wherein samples were run in triplicate. *P<0.05, **P<0.01.