Selective Processing and Metabolism of Disease-Causing Mutant Prion Proteins
Figure 3
All globular domain PrP mutants display altered localization.
Cells expressing wtPrP and the indicated mutants were stained, imaged and analyzed as in Fig. 2. The surface∶intracellular ratio of PrP is plotted for each of the mutants for comparison with wtPrP. Data points represent individual cells from a single representative experiment. All mutants were analyzed together on the same day. Horizontal black bars indicate the mean values for each data set. Each mutant dataset was compared to wtPrP by the Student's t-test and found to be statistically significant in all cases (p<10−8), except PrP(A117V).