Species-Specific Activity of SIV Nef and HIV-1 Vpu in Overcoming Restriction by Tetherin/BST2
Figure 6
Interferon induces tetherin and inhibits the release of nef-deleted SIV from an infected rhesus macaque cell line.
The rhesus macaque sMAGI cell line was cultured in the presence or absence of IFNα, and virus release was compared following infection with VSV G-pseudotyped SIV Δenv versus SIV ΔenvΔnef. (A) Treatment with IFNα upregulated the expression of tetherin on the cell surface. Cells were cultured in medium with or without 1000 U/ml IFNα, and the expression of tetherin was assessed by flow cytometry. (B) Deletion of the SIV nef gene significantly impaired virus release from infected cells in the presence of IFNα. Seventy-two hours after infection with VSV G-pseudotyped SIV Δenv and SIV ΔenvΔnef (50 ng/ml p27 eq. each), the amount of p27 released into the cell culture supernatant was determined by antigen capture ELISA.