The Epstein-Barr Virus G-Protein-Coupled Receptor Contributes to Immune Evasion by Targeting MHC Class I Molecules for Degradation
Figure 9
The ability of BILF1 homologs to downregulate MHC class I.
(A) Multiple sequence alignment of EBV-BILF1, the rhesus lymphocryptovirus CeHV15-BILF1, and KSHV-ORF74. The alignment was done with ClustalW version 1.8, and shading was done with Boxshade version 3.21, available at http://www.ch.embnet.org/software/BOX_form.html. (B) 293 or MJS cells were transfected with EBV-BILF1, CeHV15-BILF1 or KSHV-ORF74 genes in the bicistronic vector, pCDNA3-IRES-nlsGFP. At 48 hr post-transfection, surface MHC class I molecules were stained and analyzed exactly as in Fig. 1.