Migratory Dermal Dendritic Cells Act as Rapid Sensors of Protozoan Parasites
Figure 2
Three-dimensional distribution of dendritic cells within CD11c-YFP mice.
(A) Single plane images from 2P-IVM showing YFP+ dendritic cells (yellow) in ear skin at various vertical depths along the z-projection. Extracellular matrix in the dermis was detected by the SHG signals (blue). Scale bar, 49 µm. (B) Upper panels, representative images from three-dimensional reconstructions of ear skin of a CD11c-YFP mouse showing the distribution of LC and DDC in relation to SHG. Lower panel, a schematic representation of DC localization in relation to different compartments in the skin. (C) Upper and middle histograms depict numbers of YFP+ LC and DDC along the vertical depth in the epidermis and dermis (underneath basement membrane). Lower histogram shows LC and DDC density in the epidermis and dermis (between 20–50 µm). Bars represent mean±SEM numbers obtained from at least three individual mice.