Receptor-Induced Thiolate Couples Env Activation to Retrovirus Fusion and Infection
Figure 6
The SU-TM Disulfide Bond Is Not Disrupted upon Env Triggering by Receptor and Low pH
ALV-A was incubated with or without sTva at pH 8.0 for 20 min on ice and then for 30 min at 37 °C at pH 5.0 (+ acid) or pH 7.4 (− acid). Samples were neutralized, exposed to 100 mM DTT (+ DTT) or buffer (−DTT), and incubated at room temperataure (− boil) or 100 °C (+ boil) for 5 min prior to SDS-PAGE and analysis by immunoblot for TM (left panel). Immunoblots were then stripped with SDS/DTT and probed for SU using the mc8C5 mAb (right panel). Note that both antibodies are more sensitive for unreduced antigens.