Analysis of Cells Targeted by Salmonella Type III Secretion In Vivo
Figure 3
Detection of Secretion in C57BL/6 Spleen Cells
FACS data with levels of green and blue CCF2-AM fluorescence in spleen cells from mice infected with each fusion strain (A). Green fluorescence indicates CCF2-AM is present within the cells. The percentage of blue cells, positive for secretion, is shown in the lower right corner of each dot plot. The graph below shows the percentage of total spleen cells emitting blue fluorescence as detected by FACS analysis (B). Each X represents the value for one mouse infected with the indicated strain and horizontal bars represent the average value of seven mice. The * denotes samples for which the Student's t-test returned a value where p < 0.05 when compared to 14028-infected mice.