Role of Dendritic Cells in Differential Susceptibility to Viral Demyelinating Disease
Figure 6
Inhibition of Allogenic T Cell Stimulation by TMEV-Infected DCs
BM DCs were infected with TMEV in vitro for 3 d, and then varying numbers of unfixed or fixed BM DCs were cultured with 1 × 105 of naïve T cells from BALB/c mice. To study in vivo infected cells, DCs were isolated from the spleens of mock- or TMEV-infected mice at 7 dpi, then co-cultured with naïve T cells. Cells and culture supernatants were collected after 96 h of co-culture.
(A, B) T cell proliferation was measured as the mean ± SD of (3H)-thymidine uptake from triplicate cultures.
(C, D) IFN-γ and IL-4 produced in culture supernatants (DC:T = 1:10) were determined by ELISA. UD, undetectable. **, significant differences between the values in TMEV- and mock-infected cultures (p < 0.01). Data from BM DCs infected in vitro are shown in (C), and those from infected mice in (D).
(E, F) The effect of co-culture with infected DCs on the expression of CD69 or TIM-3 on T cells (DC:T = 1:10) was determined by FACS. The numbers represent the percentage of CD69- or TIM-3-positive cells relative to total CD4+ or CD8+ T cells.