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The Ebola Virus VP35 Protein Is a Suppressor of RNA Silencing

Figure 7

VP35 dsRNA-Binding Capacity Is Required for trans Complementation of HIV-rtTA-Tat^fs

HEK293T cells were transfected with HIV-rtTA-Tat^wt, HIV-rtTA-Tat^fs, and expression plasmids for Tat^wt, VP35^wt, or the various VP35 mutants (K309A, R312A, and K309/R12A), or a C-terminal deletion mutant R300T (50 ng). Virus production was determined 2 d post transfection.

doi: https://doi.org/10.1371/journal.ppat.0030086.g007