Specific Remodeling of Splenic Architecture by Cytomegalovirus
Figure 4
MCMV-Induced Suppression of CCL21 Is Independent of LTαβ-LTβR Signaling
LTα-deficient or wild-type (B6) mice were infected with MCMV (Smith strain, 3.2 × 105 pfu), and spleens were harvested at various times post-infection for analysis of CCL21 and gp38 levels by quantitative PCR. Gene expression levels displayed on the y-axis are the levels in uninfected mice, and fold reduction in gene expression in uninfected LTα−/− compared with wild-type is shown in the parentheses near the y-axis. Values in parentheses at the right of the figure represent the fold reduction in gene expression 3 d post-virus-infection compared with uninfected mice of the same genotype.
(B) B6 mice were infected with various amounts of MCMV (2 × 104, 1 × 105, 3.2 × 105 pfu), and spleens were harvested 3 d post-infection for analysis of CCL21-ser and LTβ mRNA levels by quantitative PCR. Mice were infected in parallel with the same doses of MCMV for analysis of total splenocyte numbers 3 d post-infection.