Identification of the Moving Junction Complex of Toxoplasma gondii: A Collaboration between Distinct Secretory Organelles
Figure 7
RON4 localization in ΔAMA1/AMA-myc Tachyzoites Following Tetracycline Repression and Abortive Invasion
DIC (A) and deconvolution IIF (B–D) were used to image formaldehyde-fixed cultures. Images represent a projection through ten 0.2-μm sections. Intracellular ΔAMA1/AMA-myc tachyzoites were grown in Atc-containing media for 30 hr, syringe-released and then synchronized for invasion using a potassium shift as described in the methods. After allowing invasion for 20 min, the cover-slips were fixed and processed for IIF without detergent permeabilization. SAG1 staining ([B] FITC) shows an extracellular parasite in contact with the HFF monolayer. (C) shows the RON4 (Texas Red staining). (D) is a merge of (B) and (C).