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Assessing shared respiratory pathogens between domestic (Ovis aries) and bighorn (Ovis canadensis) sheep; methods for multiplex PCR, amplicon sequencing, and bioinformatics to characterize respiratory flora

Fig 2

MLST results for Mannheimia sequences using mixed reads from closely-related species.

Numbers in data labels represent proportion of reads from each of two mixed type strain libraries. Mh = Mannheimia haemolytica; Mr = Mannheimia ruminalis; Mg = Mannheimia glucosida; Bt = Bibersteinia trehalosi. Tree produced with maximum likelihood algorithm and Mannheimia varigena reference as root.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0293062.g002