A novel metric to improve mismatched primer selection and quantification accuracy in amplifying DNA repeats for quantitative polymerase chain reactions
Fig 2
Similar PCR amplification efficiency (E) between mismatched telomere and perfect-match reference gene primers with identical oligo duplex template.
Individual PCR efficiency from each reaction containing serial dilutions of respective oligo duplex templates at 6, 0.6 and 0.06 pg using Cycling Program #1 (60°C only). (A). 36B4 and IFNB1 primer pairs with oligo duplex IFNB1-36B4-ds as template. (B). The five sets of primer pairs with oligo duplexes Tel-36B4-ds and Tel-IFNB1-ds as templates.