An atopic dermatitis-like murine model by skin-brushed cockroach Per a 2 and oral tolerance induction by Lactococcus lactis-derived Per a 2
Fig 1
Dose finding schedule of cockroach allergen Per a 2-induced allergic dermatitis via skin-painting in BALB/c mice.
Purified proteins of recombinant Per a 2 were applied epicutaneously once per week for 5 weeks. To examine the response of sensitization, scratching behavior was videoed on day 40 after the intradermal (ID) challenge. Serum samples were obtained from the submandibular vein bi-weekly and preserved at -20°C until analysis. All mice were sacrificed on the 42nd day and the ID site of the skin and spleen were excised for further study.