Divide, conquer and reconstruct: How to solve the 3D structure of recalcitrant Micro-Exon Gene (MEG) protein from Schistosoma mansoni
Fig 4
1H-15N HSQC isoforms 1 overlaid spectra.
Superimposition of 1H-15N HSQC spectra of isoform 1 (red) and A) iso 1a (blue), B) iso 1b (green), C) iso 1c (pink) and D) iso 1d yellow). The experiments were carried out at 28.2 T for isoform 1 and 14.1 T for the shortest peptides (iso 1a, iso 1b, iso 1c and iso 1d)). The samples were dissolved in DMSO, and the concentration of each peptide was set to 2 mM. The temperature was set to 27°C. Residue assignments are displayed in the spectra and the isoform 1 sequence is displayed at the top of the figure. Each sequence peptide is highlighted with a different color for the sake of clarity.