Lysophosphatidic acid down-regulates human RIPK4 mRNA in keratinocyte- derived cell lines
Fig 7
Depletion of RIPK4 mediates the effects of LPA on cell cycle progression in HaCaT and A431 cells.
A: RIPK4 was knocked down by two shRNAs in HaCaT cells. RIPK4 mRNA levels in the knockdown cells and control cells are shown; B: Cell cycle progression of control and RIPK4-knockdown HaCaT cells with or without LPA treatment was analyzed via propidium iodide staining and flow cytometry G1, S, and G2 populations were gated as indicated; C: The percentage of S/G2 population in control or RIPK4 knockdown cells was compared after LPA or vehicle (FAF-BSA) treatment. *****: p < 0.00005, student’s t test; n.s: not significant. D: RIPK4 was knocked down by two shRNAs in A431 cells. RIPK4 mRNA levels in the knockdown cells and control cells are shown; E: Cell cycle progression of control and RIPK4-knockdown A431 cells with or without LPA treatment was analyzed via propidium iodide staining and flow cytometry; F: The percentage of S/G2 population in control or RIPK4 knockdown cells was compared after LPA or vehicle (FAF-BSA) treatment. *: p < 0.05; **: p < 0.005; *****: p < 0.00005, student’s t test. Data were pooled from two independent experiments, with each condition tested in quadruplicate. Future studies will provide pathway analysis to define these pathways.