A seeding-based neuronal model of tau aggregation for use in drug discovery
Fig 7
Implementation of the tau seeding model for drug screening.
A) Diagram highlighting the key steps in the culture and treatment of primary neurons for use with the tau seeding model. Cortical neurons are cultured from hTau embryos, transduced with T7-P301S LV at DIV4 and treated with P301S lysates at DIV7. WT lysates are used as a negative control. Tau inclusions are detectable from DIV14 onwards. Treatments aimed at reducing the level of accumulated tau can be started from DIV7. The specific time-points of intervention and analysis may be defined by the end user according to experimental needs. B) Strategy for obtaining large quantities of comparable seeding material to reduce seeding variability.