A seeding-based neuronal model of tau aggregation for use in drug discovery
Fig 1
Designing a neuronal seeding-based model of tau accumulation.
A) Diagram of experimental design. B) Tau aggregation HTRF in DIV18 lysates from cells treated with control, T7-WT tau or T7-P301S tau LV at MOI 5, and lysates from WT or P301S mice at 166pg/cell, or PBS as a vehicle control. Data shown as mean ± SEM, n = 3–4 independent experiments. Assay background = 627 ± 50. One-Way ANOVA with Dunnett’s multiple comparisons test. All conditions tested against Control LV + PBS. C) Representative images from hTau primary neurons treated with T7-P301S LV and WT or P301S brain lysates. Cells were fixed at the indicated DIVs. Left: zoom images of the areas highlighted in white. D) Quantification of the number of Triton-insoluble T7 inclusions in hTau primary neurons treated as in C. Data shown as mean ± SEM, n = 3–4 independent experiments. Two-Way ANOVA with Sidak’s multiple comparisons test, comparing WT vs P301S brain lysate for each DIV.