Changes in the proteome and secretome of rat liver sinusoidal endothelial cells during early primary culture and effects of dexamethasone
Fig 8
Effect of Dex on LSEC viability in culture.
(A) Freshly isolated rat LSECs were seeded in similar densities and cultured for the indicated time points in 5% O2 and 5% CO2 in DMEM with serum-free supplements ± 1 μg/ml Dex, and/or 100 U/ml IL-1β. Cell viability was measured with a Live/Dead Cell Imaging Kit (Thermo Scientific) as described in Material and Methods. Results are presented as % of attached cells at 2 h (set as 100%) and are the average of 3 biological replicates. Error bars show SD. *Significant between groups (One-way ANOVA, Tukey HSD posthoc test, p < 0.05). (B) LSEC cultures were incubated with 0, 0.1, 1, or 10 μg/ml Dex for 24 h. Recombinant human Fas Ligand protein (rhsFasL, 100 ng/ml) was used as positive control and added to non-treated cultures 4h before the end of incubation time. Caspase 3/7 activity is shown in percent of the activity in non-treated LSECs without rhsFasL. Results are mean of 4 biological replicates; error bars show SD. *Significantly different from non-treated cultures and cultures with rhsFasL, **significantly different from non-treated cultures (one-way ANOVA, p < 0.05).